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- W2385263623 abstract "Objective To explore the expression and effect of the recombinant vector of human PAX8/ PPARγ fusion gene(PPFP) in human thyroid follicular epithelial cell Nthy ori 3-1.Methods The PAX8 gene was isolated and amplified by PCR technique from PAX8-pOTB7 plasmid.The PPARγ gene was isolated and amplified by PCR technique from PPARγ-pCMV-SPORT6 plasmid.Then the two genes were directionally united and this united gene was subcloned into the eukaryotic expression plasmid of pEGFP-C1 vector to generate the eukaryotic expression recombinant vector,pEGFP-C1-PAX8/ PPARγ.The correct PPFP gene was confirmed by PCR,edoenzyme digestion,sequencing,analysis,and contrast.After the co-transfection of pEGFP-C1-PAX8/ PPARγ and lipofectamine 2000 into human thyroid follicular epithelial cell Nthy ori 3-1,the expressions of PPFP fusion gene on the levels of mRNA and protein were detected by RT-PCR and Western blot.Results The correct eukaryotic expression recombinant vector,pEGFP-C1-PAX8/ PPARγ,was confirmed by verification.After the pEGFP-C1-PAX8/ PPARγ and Lipofectamine 2000 were transfected into Nthy ori 3-1 cells,the expressions of PPFP fusion gene on the levels of mRNA and protein were successfully detected.Conclusions The PPFP fusion gene and the eukaryotic expression recombinant vector pEGFP-C1-PAX8/ PPARγ have been constructed successfully.After this recombinant vector is transfected into Nthy ori 3-1 cells,it could successfully express PPFP protein,and provides an experimental basis for further exploring the molecular mechanisms of PPFP′s tumorigenesis." @default.
- W2385263623 created "2016-06-24" @default.
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- W2385263623 date "2009-01-01" @default.
- W2385263623 modified "2023-09-25" @default.
- W2385263623 title "Cloning of PPFP fusion gene (composed of PAX8 and PPARγ) and construction of the recombinant vector containing human PPFP gene" @default.
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