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- W2385272656 abstract "Cadmium( Cd) is a toxic transition metal with great occupational and environmental concern. Soluble cadmium salts accumulate and result in toxicity to various organs including liver,kidneys,brain,lungs and heart. A variety of adverse health effects,such as bone fracture,renal dysfunction,hypertension and cancer,can happen after the constant exposure of cadmium. However,the exact cellular mechanisms of cadmium toxicity are not yet well understood. In this study,cytotoxic effects of cadmium on Saccharomyces cerevisiae were investigated with or without some antagonists. For cadmium treatments,yeast cells of EGY48 strain harvested from the early log phase were incubated in 0. 9% NaCl containing varying amounts of cadmium chloride( Cd) for 3 to 24 h. For other combination treatments,selected antagonists including broad caspase inhibitor Z-Asp-2,6-dichlorbenzoyloxymethylketone( Z-Asp-CH2-DCB),ascorbic acid( AsA) and catalase( CAT),calcium inhibitor ethylene glycol bis-( 2-aminoethyl)-tetraacetic acid( EGTA) and LaCl3were respectively added to cadmium solutions at 0. 05 to 5 mmol·L-1,using 2',7'-dichlorofluorescein diacetate( DCFH-DA) to indicate intracellular ROS level and methylene blue staining method to measure the cell viability. The results showed that cadmium exposure decreased cell viability in a dose-and time-dependent manner. Exposure to 0. 25 mmol·L-1Cd for 6 h or exposure to 0. 5 mmol·L-1and higher concentrations of Cd for3 h significantly induced cell death. Cadmium exposure induced significant cell death and also an obvious increase of intracellular ROS levels. Caspase inhibitor Z-Asp-CH2-DCB can significantly block Cd-induced cell death. Moreover,when AsA or CAT was used to block intracellular ROS increase,Cdinduced cell death decreased significantly. Also,EGTA and LaCl3can block Cd-induced cell death. These results clearly demonstrated that Cd-induced cell death is associated with an increase of the intracellular ROS and Ca2+levels. Our results suggest that Cd-induced cell death in S. cerevisiae could be one kind of programmed cell death mediated by ROS generation and activation of cytosolic Ca2+signaling." @default.
- W2385272656 created "2016-06-24" @default.
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- W2385272656 date "2014-01-01" @default.
- W2385272656 modified "2023-09-24" @default.
- W2385272656 title "Involvement of ROS and calcium in cadmium-induced yeast cell death" @default.
- W2385272656 hasPublicationYear "2014" @default.
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