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- W2385282338 abstract "The gene encoding the surface antigen 2 of E.tenella(EtSAG2) Yangling strain was amplified by RT-PCR.The PCR product was purified and cloned into pGEM-T easy vector and the recombinant plasmid was sequenced.The EtSAG2 ORF without the signal sequence was amplified by PCR from the pGEM-SAG2 using the specific primers and cloned into the expression vector pET-32a(+),and then was transformed into E.coli BL 21(DE3).After conformed by PCR with endonuclease digestion,the cloned gene was sequenced and aligned(online) with BLAST program.The result showed the cloned sequence of EtSAG2 from E.tenella Yangling strain was highly similar to that of(E.tenella) H strain with an identity of 99.75%.The gene EtSAG2 without signal sequence in recombinant plamid pET-32a(+)-EtSAG2 could be expressed in inclusion body of E.coli BL 21 when induced by IPTG,and the molecular size of recombinant protein was 47 ku(revealed) by SDS-PAGE." @default.
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- W2385282338 date "2006-01-01" @default.
- W2385282338 modified "2023-09-25" @default.
- W2385282338 title "Cloning and expression of the gene encoding SAG2 of Eimeria tenella" @default.
- W2385282338 hasPublicationYear "2006" @default.
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