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- W2385282554 abstract "Based on an infectious molecular clone of HFV(pHSRV1), helper vector pΔGP was successfully constructed by deletion of the gag and pol genes, substitution SV40 polyA signal for the 3′LTR of human foamy virus.Replication-defective vector pGPSNI-EGFP and helper vector pΔGP were cotransfected into HIC cell line. Moreover, replication-defective vector pGPSNI-EGFP and helper vector pΔGP were transfected into HIC cell line as a control, respectively. Using fluorescence microscopy, the cotransfected HIC cell with pGPSNI-EGFP and pΔGP vectors strongly expressed green fluorescence protein(GFP) and the transfected HIC cell with replication-defective vector pGPSNI-EGFP weakly expressed green fluorescence protein, while the transfected HIC cell with helper vector pΔGP did not completely express green fluorescence protein. The result showed not only that replication-defective vector pGPSNI-EGFP and helper vector pΔGP from human foamy virus were successfully constructed, but also the 3′ end sequence of human foamy virus′ env gene(internal promoter, IP) had weak promoter activity and Bel protein promoted by IP transactivated both IP and the 5′ LTR promoter of human foamy virus strongly." @default.
- W2385282554 created "2016-06-24" @default.
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- W2385282554 date "2003-01-01" @default.
- W2385282554 modified "2023-09-25" @default.
- W2385282554 title "Study on the Transfection of Constrction Replication-DefectiveVirus Vector Helper Plasmid pΔGP to HIC Cell" @default.
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