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- W2385287567 abstract "Objective To construct prokaryotic expression vectors for Livinα and Livinβ and to obtained the fusion protein of pET32a(+)-livinα and pET32a(+)-livinβ.Methods Total RNA of Hela cell was extracted.The full-length cDNA of Livin isoforms was gained by RT-PCR.Then inserted into pET32a(+)vector and constructed the recombinant plasmids pET32a(+)-livinα/DH5α and pET32a(+)/livinβ/DH5α,sequencing was performed to guarantee correct sequence insertion for the isoforms Livinα and Livinβ.Reconstructed the recombinant plasmids pET32a(+)-livinα/BL21 and pET32a(+)/livinβ/BL21,the pET32a(+)-livinα/BL21 and pET32a(+)-livinβ/BL21 plasmids was induced after 4.5 hours by IPTG,and the value of A600nm was 0.6 in LB medium.Expression of the pET32a(+)-livinα/BL21 and pET32a(+)-livinβ/BL21 were analyzed by SDS-PAGE.Results Full-length cDNA of Livinα and Livinβ was cloned respectively and subcloned into pET32a(+)successfully.Fusion protein of positive recombinants were gained by pET32a(+)prokaryotic expression system.Conclusion The construction of prokaryotic expression vector for Livinα and Livinβ and validation of expression in cells provide basis for further research on functions of Livinα and Livinβ and their anti-apoptosis effect in cancer cell." @default.
- W2385287567 created "2016-06-24" @default.
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- W2385287567 date "2007-01-01" @default.
- W2385287567 modified "2023-09-26" @default.
- W2385287567 title "Construction of Prokaryotic Expression Vectors for Livin Alpha and Livin Beta" @default.
- W2385287567 hasPublicationYear "2007" @default.
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