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- W2385304846 abstract "Objective To investigate the effects of long-term cultivation in vitro on the proteome of keratinocytes. Methods The whole-cell proteins were prepared from the primary and the seventh-passage human skin keratinocytes and applied to two dimension gel electrophoresis (2-DE). The 2-DE patterns were analyzed by PDQuest software. The selected differentially expressed protein spots were analyzed by tandem time-of-flight mass spectrometry. The mRNA levels of two differentially expressed proteins were further detected by semi-quantitative RT-PCR. Results The 2-DE patterns with high resolution were obtained. Two differentially expressed proteins down-regulated in the seventh-passage keratinocytes were identified as psoriasis associated fatty acid binding protein (PA-FABP) and galectin-7. The results of RT-PCR suggested that the transcription levels of both PA-FABP and galectin-7 were much lower in the seventh-passage keratinocytes than those in the primary keratinocytes. Conclusion Long-term cultivation of human skin keratinocytes in vitro might affect the expression of some proteins related to fatty acid metabolism and apoptosis." @default.
- W2385304846 created "2016-06-24" @default.
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- W2385304846 date "2010-01-01" @default.
- W2385304846 modified "2023-09-28" @default.
- W2385304846 title "Effects of cultivation in vitro on the proteome of human skin keratinocytes" @default.
- W2385304846 hasPublicationYear "2010" @default.
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