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- W2385320475 abstract "Objective To investigate the effect of siRNA interfering glucose regulated protein78(GRP 78) on cisplatin-resistance of human ovarian cancer SKOV3/DDP cells,and clarify the biomechanism of the effect of GRP78 gene silencing on the cisplatin-resistance of human ovarian cancer SKOV3/DDP cells.Methods The pSilencerTM3.0-H1-GRP78 siRNA plasmid was constructed,then it was transfected into cancer cells by Lipofectamine 2 000.The expressions of GRP78 mRNA and protein were measured by RT-PCR and Western blotting.The expressions of caspase-4 protein and caspase-3 protein were detected by Western blotting.The apoptotic rate of cells was measured by flow cytometry.Results Compared with control group,the GRP78 gene and protein expression levels were markedly down-regulated following GRP78 siRNA plasmid transfection(P0.05);Compared with untransfecting GRP78 siRNA plasmid group,the protein expressions of caspase-4 and caspase-3 in SKOV3/DDP cells in GRP78 siRNA plasmid transfection group induced by cisplatin treatment were sinificantly increased(P0.05),and the apoptotic rate was also increased(P0.05).Conclusion The suppression of GRP78 gene expression can increase the apoptotic rate of SKOV3/DDP cells through up-regulating the expressions of caspase-4 and caspase-3,so the suppression of GRP78 gene expression can decrease the drug resistance of SKOV3/DDP cells to cisplatin." @default.
- W2385320475 created "2016-06-24" @default.
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- W2385320475 date "2011-01-01" @default.
- W2385320475 modified "2023-09-23" @default.
- W2385320475 title "Effect of GRP78 gene expression down-regulation on cisplatin-resistance of human ovarian cancer SKOV3/DDP cells" @default.
- W2385320475 hasPublicationYear "2011" @default.
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