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- W2385364033 abstract "【Objective】The objective of the study is to develop a sensitive detection technique on grapevine viruses and understand the phylogenetic relationships of Sichuan isolates with other geographic origins.【Method】Comparison with three different RNA extraction protocols,the Reverse Transcription-Polymerase Chain Reaction(RT-PCR) were optimized to detect three different grapevine viruses.Partial genomes of three different viruses isolated from Sichuan were cloned and the phylogenetic studies of each isolate were conducted,respectively.【Result】The results indicated high quality RNA was obtained quickly from the mature phloem of shoots by the modified silica particles extraction only.RT-PCR was successfully used to amplify partial fragments of the GLRaV-2,GVB and GVA with 18S RNA of grapevine as internal control,respectively.In addition,phylogenetic analysis and nucleotide identities studies clearly clustered the 16 CP genes of GLRaV-2 isolates collected from different geographic origins into five phylogenetic groups.The nucleotide identities of GVB CP genes between 7 isolates of distinct geographic origins were calculated to 79.1%-98.7%.Five isolates of GVA from Sichuan were clustered into 4 clades based on the phylogenetic structure,so it was suggested that the virus populations of GVA from Sichuan consisted of genetic diversity of variants.【Conclusion】The studies suggested that GLRaV-2,GVA and GVB had genetic variability at level of nucleotide acids.Furthermore,GVA populations in Sichuan had significant genetic diversity and included 4 different strains." @default.
- W2385364033 created "2016-06-24" @default.
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- W2385364033 date "2013-01-01" @default.
- W2385364033 modified "2023-09-25" @default.
- W2385364033 title "RT-PCR detections and phylogenetic studies on three viruses from grapevine" @default.
- W2385364033 hasPublicationYear "2013" @default.
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