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- W2385364191 abstract "OBJECTIVE:To study the way of culturing endothelial progenitor cells from human bone marrow,the transduction of adenoviral vectors delivering genes and the cell biological changes after the transduction.METHODS: Mononuclear cells were seperated from healthy bone marrow by gradient centrifugation,cells were cultured in vascular endothelial growth factor(VEGF165) containing medium for 2 weeks,then cells surface markers CD14,CD34,CD45,CD166,CD29,CD44,HLA-DR,CD31,CD106,CD133 and VEGFR2(KDR) were tested by flow cytometry.Adenovirus vectors of enhanced green fluorescent protein gene(Ad-EGFP) were used to test the adenovirus vectors infecting efficiency to EPCs.hγIFN secreted by EPCs-hγIFN was tested by ELISA.MTT was used to test the cell growth ability of EPCs,EPCs-EGFP and EPCs-hγIFN.Markers CD133,KDR and CD34 of EPCs-EGFP and EPCs-hγIFN were tested by cell cytometry.RESULTS:When the mononuclear cells separated from bone marrow cultured in VEGF165 containing medium 2 weeks later,the adherent cells expressed EPCs specific surface markers CD133,KDR and CD34.When Ad-EGFP transduced EPCs at multiplicity of infection(MOI) at 150 pfu/EPCs,GFP was positive in more than 90% of EPCs two days later.Ad-hγIFN transduced EPCs(EPCs-hγIFN) could secret high and stable hγIFN.The EPCs growth changed little after the transduction,and CD133,KDR and CD34 positive rate of EPCs,EPCs-EGFP,EPCs-hγIFN were unchanged. CONCLUSIONS: EPCs can be cultured from bone marrow,adenoviral vectors delivering genes could transfect EPCs efficiently.The growth and markers of EPCs were unchanged after the transduction." @default.
- W2385364191 created "2016-06-24" @default.
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- W2385364191 date "2012-01-01" @default.
- W2385364191 modified "2023-09-23" @default.
- W2385364191 title "Culture of endothelial progenitor cells and the transduction of adenovirus vectors delivering genes" @default.
- W2385364191 hasPublicationYear "2012" @default.
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