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- W2385382256 abstract "To obtain the purified recombinant protein used for serologic diagnosis of dengue virus infection, the DⅢ region of dengue virus type Ⅰ envelope gene was amplified from Hawaii strain by reverse transcription nested PCR. The PCR products were ligated with plasmid pEU-30a and transformed into E.coli competent cells BL21(DE3) to express recombinant protein,and the expressed products were identified and purified by Western-blot and electroelution. The purified recombinant protein was applied to test serum from patients infected with dengue virus by indirect ELISA and capture ELISA. The results were compared with those tested by IFA. Result of this study showed that the recombinant protein was expressed with high yields in E.coli. The result of Western-blot indicated that it possessed good antigenic activity. Serum from patients infected with dengue virus type Ⅰ can be highly detected by recombinant protein. It is concluded that dengue virus type Ⅰ envelope gene DⅢ region has been successfully expressed in E.coli and it can be used as an in antigen for the detection.of dengue virus infection." @default.
- W2385382256 created "2016-06-24" @default.
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- W2385382256 date "2006-01-01" @default.
- W2385382256 modified "2023-09-25" @default.
- W2385382256 title "Cloning and expression of the DIIIregion of dengue virus type I envelope gene region and application of the recombinant protein" @default.
- W2385382256 hasPublicationYear "2006" @default.
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