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• W2385553184 abstract "Objective:Fe3O4 magnetic microspheres were prepared by solvothermal reduction method and coated with amorphous silica using conventional sol-gel method.The silica coated magnetic microspheres were used as solid phase adsorbents for extraction of genomic DNA from castor leaves.Methods:The characterizations of the magnetic microspheres were carried on transmission electron microscopy(TEM),fourier transform infrared(FTIR)spectro-scopy,vibrating sample magnetometer(VSM),and then the separated DNA was verified with agarose gel electrophoresis.Results:The silica coated magnetic microspheres were spherical in morphology with superparamagnetic characteristics and high magnetic saturation.The A260/A280 ratio of DNA samples isolated using silica coated magnetic micrspheres was 1.83 and the adsorption capacity of Genomic DNA reached 0.556 mg·g-1.Conclusions:Compared with the traditional chloroform isoamyl alcohol extraction method,the process of DNA extraction using silica coated magnetic microspheres is convenient,fast and efficient." @default.
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• W2385553184 date "2010-01-01" @default.
• W2385553184 modified "2023-09-25" @default.
• W2385553184 title "Synthesis of silica coated magnetic microspheres for genomic DNA extraction from castor leaves." @default.
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