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- W2385599903 abstract "Objective To establish a HPLC method for the content determination of icariin, notoginsenside R1,ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 in Qianj in She n'anning capsule by using core-shell chromatographic column. Methods The chromatographic separation was performed on Kinetex C18column(0.46 cm×10 cm, 2.6 μm) using a mobile phase of acetonitrile(A)and water(B)at a flow rate of 1.0 m L/min. The gradient elution mode was as follows:0~6 min,19 %A;6~13min,19 %~29 %A;13~18 min,29 %~40 %A;18~25 min,40 %~19 %A. The detection wavelength and column temperature were set at 203 nm and 30℃. The injection volume was 20 μL.Results The linear range of icariin, notoginsenside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1 were 3.2 ~32 μg· m L-1(r=0.99985),3.2~32μg·m L-1(r=0.99975),11.0~110.0μg·m L-1(r=0.99995),6.0~60.0 μg·m L-1(r=0.99995),11.0~110.0μg·m L-1(r=0.99985),respectively. The average recovery was 98.82 %(RSD=0.46 %),97.08 %(RSD =1.21 %),99.64 %(RSD=0.13 %),98.69 %(RSD=1.38 %)and 98.68%(RSD=2.44 %),respectively. Conclusion The established method is simple,accurate and repeatable,and can be used for the quality control of Qianj in She n'anning capsule." @default.
- W2385599903 created "2016-06-24" @default.
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- W2385599903 date "2014-01-01" @default.
- W2385599903 modified "2023-09-25" @default.
- W2385599903 title "Establishment of Method for Determination of Five Components in Qianjin Shen'anning Capsule" @default.
- W2385599903 hasPublicationYear "2014" @default.
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