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- W2385619315 abstract "Objective To study the effect of γ-rays on the transcription of Tob1 gene in human peripheral blood lymphocytes.Methods Lymphocytes at logarithmic growing phase were irradiated at 1,2,3,4,5 and 6 Gy respectively by γ-rays.RNA were extracted 12,24 hours after irradiation and also extracted 4,8,12,24,36,48 and 72 hours after irradiation at 2 Gy dose group.RNA became cDNA through inverse transcription.Fluorescent quantitative PCR was used to detect the expression of Tob1 mRNA.Results Compared with the control,the levels of Tob1 mRNA in lymphocytes were increased at 4,24,36 hours in the 2 Gy dose group,showing 1.99 times higher than those of the control(P0.05).In the 1～5 Gy dose groups irradiated at 24 hours,the levels of Tob1 mRNA expression were increased with the doses increasing(P0.05).With the control,there were no significant changes at 12 hours in 1～5 Gy dose groups (P0.05),but the expression level in 6 Gy was upmost to 16.37 times higher than that of the control (P0.05).Conclusion The expression level of Tob1 mRNA has a dose-and time-dependant relationship after γ-rays radiation.Tob1 may become a molecular biologic dose biomarker of people receiving radiation in nuclear accident." @default.
- W2385619315 created "2016-06-24" @default.
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- W2385619315 date "2009-01-01" @default.
- W2385619315 modified "2023-09-26" @default.
- W2385619315 title "Effect of γ-rays on the transcription of Tob1 gene in human lymphocytes" @default.
- W2385619315 hasPublicationYear "2009" @default.
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