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- W2385626012 abstract "Objective:To investigate the specificity of application of RNA interference(RNAi) technology for gastric carcinoma gene therapy and the mechanism of action.Methods:Small interference RNA targeting COX-2 gene were designed.pTZU6+1 siRNA COX-2 vector was constructed and transfected into SGC-7901 cells to induce RNAi.The changes of COX-2 gene and protein expression were detected with RT-PCR and Western blot.Tumor cell proliferation and cell cycle were detected by MTT and flow cytometry.Expression of p53,PCNA,and ki67 was tested by Western blot.Results:The cell proliferation was significantly inhibited in vitro after transfection with recombinant plasmid pTZU6+1-siRNA-COX-2 into SGC-7901 cells.The cell cycle distribution was altered.The proportion of cells in G_1/G_0 phase significantly decreased and the proportion in S phase significantly increased.The mRNA level of COX-2 was down-regulated by 88.36% and 86.24% and the level of COX-2 protein was down-regulated by 94.27% and 91.59% compared with control.The expression of p53 protein was up-regulated.However,the expressions of PCNA and ki67 protein were down-regulated.All these parameters had no significant changes in control group.Conclusion: siRNA significantly inhibits the COX-2 gene expression and proliferation of SGC-7901 cells and causes the change of cell cycle distribution,which was related with up-regulation of p53 protein and down-regulation of PCNA and ki67 protein." @default.
- W2385626012 created "2016-06-24" @default.
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- W2385626012 date "2006-01-01" @default.
- W2385626012 modified "2023-09-26" @default.
- W2385626012 title "COX-2 targeted RNA interference inhibits growth of gastric carcinoma cells and induced changes of related signal molecules" @default.
- W2385626012 hasPublicationYear "2006" @default.
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