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- W2385655442 abstract "Objective To develop PCR assays for detecting Porphyromonas gingivalis (Pg) and for understanding genotype difference of Pg strains in subgingival plaque samples from chronic periodontitis patients. Methods Culture method was used to isolate Pg strains were isolated in subgingival plaque samples from different foci of chronic periodontitis. Pg 16S rDNA gene, collagenase gene (prtC) and fimbrillin gene (fimA) of Pg strains in these samples were detected by PCR. Partial amplification products were sequenced after T-A cloning. Results Among 122 subgingival plaque samples from 61 patients, the positive rates of Pg 16S rDNA, prtC and fimA gene detection were 90.6%, 81.9% and 78.0% respectively. In the same samples, the positive rate of co-presence of the three genes was as high as 98.4% while the positive rate was lower as 31.1% by routine culture. 18 of 60 patients (30.0%) were found to be infected with different genotypes of Pg strains in different tooth sites of an individual. The homology of PCR products from Pg 16S rDNA, prtC and fimA genes ranged from 98.62% to 100% compared with the reported nucleotide sequences. Conclusion The PCR assays developed in this study show high sensitivity and specificity, and are suitable for clinical fast diagnosis of Pg in subgingival plaque samples from chronic periodontitis patients. Some patients can be simultaneously infected by Pg different genotypes of Pg strains." @default.
- W2385655442 created "2016-06-24" @default.
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- W2385655442 date "2002-01-01" @default.
- W2385655442 modified "2023-09-25" @default.
- W2385655442 title "Detection of Porphyromonas gingivalis from subgingival plaques of patients with chronic periodontitis PCR and genotype analysis of responssible strains" @default.
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