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- W2385702965 abstract "Objective To investigate the effect of docosahexaenic acid(DHA) on the growth of human gallbladder carcinoma GBC-SD cell. Methods By cells culturing in vitro,the human gallbladder carcinoma GBC-SD cells of logarithmic growth phase was cultured in RPMI1640 medium supplemented with DHA at concentration 12.5ug/ml、25 ug/ml、50ug/ml respectively.After incubation from 24h to 72h,cell viability and proliferation was determined by MTT colourimetric assay,the cellular morphology was observed through microscope.Agarose gel electrophoresis and stained by Annexin V-FITC/PI fluorescence microscopy were used to detect apoptosis.Results After incubation with 12.5ug/ml DHA for 24h,48h and 72h,the absorbance of GBC-SD cells was not affected,the cellular morphology was not changed significantly through microscope.After incubation with 25 ug/ml、50ug/ml DHA for 24h,48h and 72h,the absorbance of GBC-SD cells was inhibited significantly,cell shrinkage and chromatin condensation could be seen through microscope.After incubation with25 ug/ml、50 ug/ml DHA for 24h,a DNA ladder was appeared when ran on an agarose gel.After incubation with25ug/ml、50 ug/ml DHA for 24h,GBC-SD cell stained by Annexin V-FITC/PI,more green fluorescence could be seen through fluorescence microscopy.Conclusions DHA can inhibit the growth of human gallbladder carcinoma GBC-SD cell line and induce the apoptosis." @default.
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- W2385702965 date "2009-01-01" @default.
- W2385702965 modified "2023-09-24" @default.
- W2385702965 title "The inhibitory effect of docosahexaenic acid on the growth of human gallbladder carcinoma GBC-SD cell line" @default.
- W2385702965 hasPublicationYear "2009" @default.
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