Matches in SemOpenAlex for { <https://semopenalex.org/work/W2385721449> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2385721449 endingPage "6" @default.
- W2385721449 startingPage "193" @default.
- W2385721449 abstract "To determine the effects of exogenous RASSF1A gene on the proliferation and expression of P65 and subunit of NF-kappaB, in lung adenocarcinoma cell line A549.pcDNA3.0-RASSF1A and pcDNA3. 0 were introduced into A549 cell line by lipofectin transfection, and the A549 cells stably expressing RASSF1A gene were established by G418 selection. The expression of RASSF1A was detected by Western blotting. The cytobiologic characterizations of the positive clone were analyzed by methythiazoletertraolium (MTT) assay and cytometry. The expressing of P65 was analyzed by RT-PCR and Western blotting.A549 cells stably expressing RASSF1A protein were established by lipofection mediated transfection and selected for further study. Compared with the nontransfected and vector transfected cells, the positive clone cells grew more slowly. Flow cytometric data showed that more positive clone cells went into phase G0/G1 and fewer cells went into phase S. The expression of P65 in nuclear protein in positive clone cells was lower than that of the control group while there was no obvious difference between the expression of p65 mRNA and P65 protein in total protein among the 3 groups.RASSF1A gene might suppress the proliferation of A549 cells through blocking the activity of P65 protein." @default.
- W2385721449 created "2016-06-24" @default.
- W2385721449 creator A5032726728 @default.
- W2385721449 creator A5045602137 @default.
- W2385721449 creator A5047661456 @default.
- W2385721449 creator A5069331173 @default.
- W2385721449 creator A5069689932 @default.
- W2385721449 date "2005-04-01" @default.
- W2385721449 modified "2023-09-27" @default.
- W2385721449 title "[Proliferation inhibition of human lung adenocarcinoma cell line A549 transfected by RASSF1A gene]." @default.
- W2385721449 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15898433" @default.
- W2385721449 hasPublicationYear "2005" @default.
- W2385721449 type Work @default.
- W2385721449 sameAs 2385721449 @default.
- W2385721449 citedByCount "1" @default.
- W2385721449 countsByYear W23857214492017 @default.
- W2385721449 crossrefType "journal-article" @default.
- W2385721449 hasAuthorship W2385721449A5032726728 @default.
- W2385721449 hasAuthorship W2385721449A5045602137 @default.
- W2385721449 hasAuthorship W2385721449A5047661456 @default.
- W2385721449 hasAuthorship W2385721449A5069331173 @default.
- W2385721449 hasAuthorship W2385721449A5069689932 @default.
- W2385721449 hasConcept C104317684 @default.
- W2385721449 hasConcept C153911025 @default.
- W2385721449 hasConcept C29537977 @default.
- W2385721449 hasConcept C54009773 @default.
- W2385721449 hasConcept C54355233 @default.
- W2385721449 hasConcept C553184892 @default.
- W2385721449 hasConcept C62112901 @default.
- W2385721449 hasConcept C81089528 @default.
- W2385721449 hasConcept C81729549 @default.
- W2385721449 hasConcept C81885089 @default.
- W2385721449 hasConcept C85265743 @default.
- W2385721449 hasConcept C86803240 @default.
- W2385721449 hasConceptScore W2385721449C104317684 @default.
- W2385721449 hasConceptScore W2385721449C153911025 @default.
- W2385721449 hasConceptScore W2385721449C29537977 @default.
- W2385721449 hasConceptScore W2385721449C54009773 @default.
- W2385721449 hasConceptScore W2385721449C54355233 @default.
- W2385721449 hasConceptScore W2385721449C553184892 @default.
- W2385721449 hasConceptScore W2385721449C62112901 @default.
- W2385721449 hasConceptScore W2385721449C81089528 @default.
- W2385721449 hasConceptScore W2385721449C81729549 @default.
- W2385721449 hasConceptScore W2385721449C81885089 @default.
- W2385721449 hasConceptScore W2385721449C85265743 @default.
- W2385721449 hasConceptScore W2385721449C86803240 @default.
- W2385721449 hasIssue "2" @default.
- W2385721449 hasLocation W23857214491 @default.
- W2385721449 hasOpenAccess W2385721449 @default.
- W2385721449 hasPrimaryLocation W23857214491 @default.
- W2385721449 hasRelatedWork W2354191045 @default.
- W2385721449 hasRelatedWork W2357329258 @default.
- W2385721449 hasRelatedWork W2360960154 @default.
- W2385721449 hasRelatedWork W2368293560 @default.
- W2385721449 hasRelatedWork W2369499945 @default.
- W2385721449 hasRelatedWork W2380688255 @default.
- W2385721449 hasRelatedWork W2384559889 @default.
- W2385721449 hasRelatedWork W2385721449 @default.
- W2385721449 hasRelatedWork W2386743395 @default.
- W2385721449 hasRelatedWork W2422013801 @default.
- W2385721449 hasVolume "30" @default.
- W2385721449 isParatext "false" @default.
- W2385721449 isRetracted "false" @default.
- W2385721449 magId "2385721449" @default.
- W2385721449 workType "article" @default.