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- W2385739811 abstract "Objective To investigate cell apoptosis induced by rapamycin on human hepatocellu- lar carcinoma BEL-7402 cells and its action mechanism.Methods BEL-7402 cells in culture medium in vitro were treated with rapamycin with different concentrations of 5,10,20,30,40 and 50 nmol/L. Cell apoptosis was detected by flow cytometry(FCM)at 24,48 and 72 h,and morphological changes were observed by Hoechst 33258 staining;The protein expression of Caspase-3,Bcl-2,Bcl-x1 and Bax was assayed by Western blotting,the activity of Caspases was determined by Caspase colorimetric as- say kit,and cell mitochondrial membrane potential was investigated by using JC-1 dying standard methods.Results Rapamycin could induce apoptosis of hepatocellular carcinoma BEL-7402 cells sig- nificantly in a time- and dose-dependent manner.Marked morphological changes of cell apoptosis were observed very dearly by Hoechst 33258 staining.Rapamycin could activate Caspase-3 and cause dis- ruption of the mitochondrial membrane potential during apoptosis.Anti-apoptotic proteins Bcl-2 and Bcl-x1 were down-regulated and pro-apoptotic protein Bax was up-regulated remarkably when apoptosis occurred.Conclusion Rapamycin could induce apoptosis by down-regulation of anti-apoptotic proteins Bcl-2,Bcl-x1 and bax up-regulation of pro apoptotic protein,as well as via disruption of mitochondrial membrane potential and activation of Caspase-3." @default.
- W2385739811 created "2016-06-24" @default.
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- W2385739811 date "2006-01-01" @default.
- W2385739811 modified "2023-09-23" @default.
- W2385739811 title "Apoptosis of hepatocelluar carcinoma BEL-7402 cells in vitro induced by rapamycin and its action mechanism" @default.
- W2385739811 hasPublicationYear "2006" @default.
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