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- W2385753224 abstract "The meso-tetra(α,α,α,α-O-phenylacetylbenzene)porphyrin was used as a complete antigen to elicit monoclonal antibody 1F2 through the immunization and cell fusion techniques. The antibody could combine with Mn porphyrin and showed a haloperoxidase activity. The affinities of the antibody for antigen and Mn porphyrin were 4 98×10 -5 mol/L and 7 63×10 -5 mol/L, respectively. When the antibody-Mn porphyrin complex was used to catalyze the halogenation reaction, the Michaelis constant and reactive rate constant κ cat for H 2O 2 were 5 127 0×10 -3 mol/L and 0 483 4 s -1, respectively. The enzyme exchange number κ cat/K m=94 285 1 mol/(L·s)." @default.
- W2385753224 created "2016-06-24" @default.
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- W2385753224 date "2001-01-01" @default.
- W2385753224 modified "2023-09-25" @default.
- W2385753224 title "Haloperoxidase by Imitation II.The Enzymological Properties" @default.
- W2385753224 hasPublicationYear "2001" @default.
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