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- W2385851808 abstract "Objective Effect of hydrogen peroxide(H_2O_2) and glutamate on the expression of apurinic/apyrimidimic endonuclase/redox factor 1(APE/ref-1) were observed,while the molecular mechanism of the nervous cells injured by glutamate was investigated.Methods As nervous cell model,PC12 cells were treated with 200 and 400 μmol/L of H_2O_2, or 10 and 20 mmol/L of glutamate,and the injury of PC12 cells was assessed by MTT. The expression of APE/ref-1 in PC12 cells was detected by Western blot.Results 200 and 400 μmol/L of H_2O_2 induced the expression of APE/ref-1 in PC12 cells,but 10 and 20 mmol/L of glutamate had no effect on the expression of APE/ref-1 in PC12 cells.Conclusion The level of oxidative stress in PC12 cells injured by glutamate is so low that the adaptive expression of anti-oxidative APE/ref-1 protein can not be induced." @default.
- W2385851808 created "2016-06-24" @default.
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- W2385851808 date "2007-01-01" @default.
- W2385851808 modified "2023-09-25" @default.
- W2385851808 title "Different response of APE/ref-1 in PC12 cell damaged by hydrogen peroxide and glutamate" @default.
- W2385851808 hasPublicationYear "2007" @default.
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