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- W2385852529 abstract "Objective:To research the effect of Bmi-1 gene on glioma cells apoptosis.Methods:Bmi-1 gene in U251 glioma cells is silenced by siRNA,and the effect was detected by RT-PCR,the apoptosis condition was detected by flow cytometry,and one way ANOVA and t test were used for statistics analysis.Results:Gel electrophoresis strip of Bmi-1 mRNA in RNA interfered U251 cells is weakly positive,those of negative controls and non-disposals are strongly positive.The gray scale ratio is statistically analyzed,and the difference between RNA interfered cells and non-interfered cells was significant.RNA Interfering may significantly down-regulate the expression of Bmi-1 in U251 cells The apoptosis rate of(32.53±6.33)% in interfered U251 cells is higher than that of(21.91±5.63)% in controls,and the difference is significant statistically.Conclusion:The Bmi-1 gene expression could inhibit the apoptosis of glioma cells and promote the genesis and development of glioma." @default.
- W2385852529 created "2016-06-24" @default.
- W2385852529 creator A5026230096 @default.
- W2385852529 date "2011-01-01" @default.
- W2385852529 modified "2023-09-22" @default.
- W2385852529 title "The preliminary study of apoptosis in glioma cells silenced Bmi-1 gene by siRNA" @default.
- W2385852529 hasPublicationYear "2011" @default.
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