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- W2385862743 abstract "Objective To explore the effect of antioxidants on the isolation and culture of adult rat hippocampal NG2 proteoglycan expressing progenitor cells (NG2 cells). Methods The primary culture was done according to the reference for isolation of NG2 cells from the adult rat hippocampus. Instead of B27 supplement, B27-AO supplement (a derivative of B27 supplement that lacks all the antioxidants) was used, and four types of antioxidants were added to B27-AO culture conditons separately or combined. Cell activities were determined by Lactate dehydrogenase (LDH) assay, and the characteristics of the cells were identified by double immunofluorescence stain. Results B27-AO supplement failed to expand or passage most of the NG2 cells, and catalase rescued most of the cells in B27-AO culture condition. Conclusion Antioxidants are essential for the isolation and culture of NG2 cells." @default.
- W2385862743 created "2016-06-24" @default.
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- W2385862743 date "2009-01-01" @default.
- W2385862743 modified "2023-09-23" @default.
- W2385862743 title "The effect of antioxidants on the isolation and culture of adult rat hippocampal neural progenitor cells" @default.
- W2385862743 hasPublicationYear "2009" @default.
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