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- W2385909062 abstract "To clone mshA gene and construct its prokaryotic expression system,and to identify immunoreactivity of the target recombinant protein(rMSHA).The mshAgene was amplified by high fidelity PCR and then T-A cloned into the new type of prokaryotic expression system pET-28a(+)-mshA-E.coliBL21DE3.The output of rMSHA was measured by SDS-PAGE and Ni-NTA affinity chromatograthy was performed to collect rMSHA.The immunity of rMSHA was determined by Western blot.DNA sequence analysis showed that the nucleic acid sequence of the cloned mshA gene was identical to that published in GenBank.The output of rMSHA expressed was as high as 20% or above of the total bacterial proteins.A 20kD fusion protein was detected by SDS-PAGE and its his-tag was recognized by its MAb in Western blot.It is evident that the fusion protein rMSHA of Vibrio cholerea O139 is well expressed in prokaryotic expression system,with fine immunoreactivity." @default.
- W2385909062 created "2016-06-24" @default.
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- W2385909062 date "2006-01-01" @default.
- W2385909062 modified "2023-09-23" @default.
- W2385909062 title "Cloning,expression and immunoreactivity of mshA gene of Vibrio cholerea O139" @default.
- W2385909062 hasPublicationYear "2006" @default.
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