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- W2385922605 abstract "The preparation of soluble yeast(1→3)-β-D-glucan phosphate by phosphorylation was investigated.The molecular structure of the glucan and its phosphate derivative was analyzed by FT-IR and 13C NMR.The immunological activity was determined by the infectious disease experiment.The effects of reaction conditions including the dosage of urea,reaction time,reaction temperature and proportioning ratio of dimethyl sulfoxide(DMSO) to H3PO4 on the phosphorylation were studied.The optimal parameters of the preparation of(1→3)-β-D-glucan phosphate were as follows: urea 15 g,reaction time 5 h,reaction temperature 100 ℃ and proportioning ratio(volume ratio) of DMSO to H3PO4 11 ∶ 4.Under these conditions,the conversion yield reached 80%,the degree of the substitution of the phosphorylated product was 0.055,and the solution 122.8 mg/mL.The FT-IR and 13C NMR spectra indicate that the phosphate groups are successfully introduced into the C2,C4 and C6 position,however the phosphate group at C4 position is limited.The glucan phosphate immunoprophylaxis significantly increases the resistance of mice to challenge with E.coli." @default.
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- W2385922605 date "2012-01-01" @default.
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- W2385922605 title "Preparation of soluble(1.RAR.3)-.BETA.-D-glucan phosphate by phosphate esterification" @default.
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