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- W2385933833 abstract "Objective:To investigate the suitable extract technology for extracting the total polysaccharides and two active components from root of Isatis indigotica.Methods:Orthogonal experiment was carried out using a three factors and three levels design to investigate the effects of temperature,water volume and extracting times on the yield,sugar content,molecular weight and monosaccharide ratio of the total polysaccharides.The sugar content was determined by phenol-sulfuric acid method.Monosaccharide compositions were analyzed with the alditol acetates by gas chromatography.Molecular weights of polysaccharides were determined by gel filtration chromatography.Results and Conclusion:The orthogonal experiments showed that the three influent factors were in this sequence:extract temperature ratio of solid to liquid extract frequency.The extract temperature was the most important factor.When the extract temperature was raised gradually,the yields,large molecular polysaccharides and the ratios of glucose in the total polysaccharides were significantly increased.However,the two active polysaccharides were markedly less at extract temperature ≥70 ℃.The optimal conditions were that ratio of solid to liquid was 1∶15,extract temperature was ≤60 ℃,and extract frequency was two times.This extract technology is economical and in favor of two active polysaccharides." @default.
- W2385933833 created "2016-06-24" @default.
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- W2385933833 date "2013-01-01" @default.
- W2385933833 modified "2023-09-26" @default.
- W2385933833 title "Extract technology of total polysaccharides and active components effect from root of Isatis indigotica" @default.
- W2385933833 hasPublicationYear "2013" @default.
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