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- W2385935524 abstract "To get a large quantity of human glucagon-like peptide-1,different hGLP-1 multim- ers containing different copies of rhGLP-1 were constructed.The synthetic hGLP-1 cDNA gene was inserted into pET-32a(+) to get the recombinant plasmid pET32-GLP-1,which could ex- press a fusion protein including thioredoxin,hexahistidine,and rhGLP-1.The coding sequence for the fusion protein was repeatedly inserted into the vector in tandem to get pET32-GLP-1-2 and pET32-GLP-1-3 with two or three copies,respectively.Three different plasmids were trans- formed into E.coli BLR (DE3) and induced by IPTG.The results demonstrated that the three kinds of E.coli ceils expressed the desired protein and the percentages of the desired protein in- creased with the numbers of gene copy.The purified rhGLP-1 showed obvious biological activity of lowering plasma glucose." @default.
- W2385935524 created "2016-06-24" @default.
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- W2385935524 date "2006-01-01" @default.
- W2385935524 modified "2023-09-26" @default.
- W2385935524 title "Cloning and Expression of rhGLP-1 Multimers in E.coli" @default.
- W2385935524 hasPublicationYear "2006" @default.
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