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- W2385946237 abstract "Objective To explore in vitro selection and mechanism of resistance to azithromycin in mycoplasma pneumoniae(MP).Methods Macrolide-resistant MP was selected in vitro from the susceptible reference strain FH,in subinhibitory concentrations of azithromycin.Antibiotic susceptibility test was porformed to identify macrolide-resistant strains through their minimal inhibitory concentration(MIC) of erythromycin.MIC of azithromycin and josamycine in macrolide-resistant strains were detected.Domain V of 23S rRNA and ribosomal protein L4 gene and L22 gene,which were associated with macrolide resistance,were amplified by polymerase chain reaction followed by direct automatic sequencing method.The DNA sequences were compared to the corresponding sequences of MP M129 to find molecular mechanisms of drug resistance.Results Eight serial(YD1-YD8) passages were selected in vitro from FH strain.The YD1-YD4 passages were susceptivity strains,the YD5-YD8 passages were macrolide resistance.MIC of these resistant strains to erythromycin azithromycin and josamycine were elevated.There were no gene mutation of domain V of 23S rRNA in the FH strain and selected passages YD1-YD6.One point mutation in domain V of 23S rRNA,A2067G,was selected in passages YD7 and YD8.Two point mutations in ribosomal protein L4,C162A and A430G,were found in all the strains,respectively.Two point mutations in ribosomal protein L22,T279C and T508C,were found in all the strains respectively.Conclusions The target mutation in domain V of 23S rRNA may be the predominant mechanism that contribute to the macrohde resistance.There is possibility to induce macrolide-resistant MP in vivo during using maerolide drugs for therapy." @default.
- W2385946237 created "2016-06-24" @default.
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- W2385946237 date "2010-01-01" @default.
- W2385946237 modified "2023-10-14" @default.
- W2385946237 title "In Vitro Selection and Mechanism of Resistance to Azithromycin in Mycoplasma Pneumoniae and Its Mechanism of Resistance" @default.
- W2385946237 hasPublicationYear "2010" @default.
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