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- W2385969892 abstract "To detect the antitumor effects of extracellular proteins produced by endophyte Bacillus subtilis DNN7 and HSN2fromLaminaria Japonica Aresch,the thalli of endophyte bacteria DNN7 and HSN2were separated from their fermentation broth by the 0.22μm filter membrane.The proteins in the fermentation broth were precipitated by ammonium sulfate and analyzed by SDS-PAGE.The antitumor activities of extracellular proteins were tested by shrimp toxicity and MTT assay.Results indicated that the extracellular proteins of DNN7 were mainly composed of three kinds of protein.The extracellular proteins of HSN2 were mainly composed of 52×103 and 44×103 two kinds of protein.Extracellular proteins of DNN7 showed a strong inhibitory activity to chronic myeloid leukemia cancer cell K562,hepatoma cell HepG2,and breast cancer cell MDA-MB-231 in vitro.The highest inhibition rate was 96.46%,42.99% and 85.70%respectively.Extracellular proteins of HSN2 also exhibited a strong inhibitory activity to chronic myeloid leukemia cancer cell K562,hepatoma cell HepG2,breast cancer cell MDA-MB-231 in vitro.The highest inhibition rate was 92.65%,66.80% and 88.48% respectively." @default.
- W2385969892 created "2016-06-24" @default.
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- W2385969892 date "2015-01-01" @default.
- W2385969892 modified "2023-09-26" @default.
- W2385969892 title "Antitumor Activity of Extracellular Proteins Produced by the Endophyte Bacillus subtilis DNN7 and HSN2 from Laminaria japonica Aresch" @default.
- W2385969892 hasPublicationYear "2015" @default.
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