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- W2386006346 abstract "A HCV/C+E 1 DNA fragment encoding core and envelope(E 1)proteins was inserted into a green fluorescent protein fusion vector to construct an eukaryotic expression recombinant plasmid,which has been transfected into mouse myeloma cell line SP2/0 by FuGENE TM 6 transfection reagent in vitro,and the green fluorescent emited by UV in SP2/0 cells was observed.It indicated the successful expression of aim genes.After screening with G 418 ,the transfected SP2/0 cells were cultured in a large amount as the target cell in detection of CTL activity.The result of cytotoxicity test suggested that EGFP could be made as a screening marker to apply to the preparation of target cell in detection of CTL activity." @default.
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- W2386006346 date "2002-01-01" @default.
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- W2386006346 title "Application of green fluorescent protein fusion vector in detecting cellular immune response" @default.
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