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- W2386071116 abstract "Objective To culture the osteoblast of Wistar rat in vitro and study its biological characteristics. Methods With heat-cool alternative method and by way of the trysin digestion, the osteoblast cells, obtained from femoral bone of Wistar rats, were colleced and cultured with special cultural fluid in vitro. After about two weeks, cells lined up in one layer. Then cells were generationed and examined by phase contrast microscope, H E strain, ALP strain, type Ⅰ and type Ⅲ collagen strains. Results The cells had the same morphological feature with ALP activity, type Ⅰ collagen strain positive. Conclusion The cultured osteoblasts derived from bone mass were seed cells for the bony tissue engineering, which is the base for the tissue-engineering born construction in vitro." @default.
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- W2386071116 date "2004-01-01" @default.
- W2386071116 modified "2023-09-28" @default.
- W2386071116 title "Culture and preliminary study of osteoblast of wistar rat in vitro" @default.
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