Matches in SemOpenAlex for { <https://semopenalex.org/work/W2386092019> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2386092019 abstract "Objective To observe the effects of different concentrations of TNF-α on the protection,inflammation and apoptosis of A549 cells.Methods A549 cells were treated with different concentrations of TNF-α(0,1,10,25 and 50 ng/mL) for six hours respectively.The cell viability was observed by MTT assay.Reactive oxygen species(ROS) levels were detected by applying ROS fluorescence probe DCFH-DA.The gene expressions of Nrf-2 and NF-κB were detected by RT-PCR.The cells were treated by nuclear fluorescence staining DAPI,and the apoptosis was observed by fluorescence microscope.Results TNF-α at the concentration of 0,1,10,25 and 50 ng/mL had no effect on A549 cell viability(all P0.05),and compared with the control group,the cell viability was decreased at the concentration of 100 ng/mL(P0.05).With the increase of TNF-α concentration,the generation of ROS increased(all P0.05),the mRNA expression of Nrf-2 decreased(all P0.05),the mRNA expression of NF-κB increased(all P0.05),and the apoptosis rate was on the rise.Conclusions Different concentrations of TNF-α can make A549 cells to produce different amounts of ROS and cellular effects.With the increase of TNF-α concentration,the cell damage is aggravated.Its mechanism may be related to the increasing amount of ROS induced by the increasing TNF-α concentration." @default.
- W2386092019 created "2016-06-24" @default.
- W2386092019 creator A5086547316 @default.
- W2386092019 date "2013-01-01" @default.
- W2386092019 modified "2023-09-24" @default.
- W2386092019 title "Effects of different concentrations of tumor necrosis factor-α on A549 cells" @default.
- W2386092019 hasPublicationYear "2013" @default.
- W2386092019 type Work @default.
- W2386092019 sameAs 2386092019 @default.
- W2386092019 citedByCount "0" @default.
- W2386092019 crossrefType "journal-article" @default.
- W2386092019 hasAuthorship W2386092019A5086547316 @default.
- W2386092019 hasConcept C121332964 @default.
- W2386092019 hasConcept C1491633281 @default.
- W2386092019 hasConcept C153911025 @default.
- W2386092019 hasConcept C16685009 @default.
- W2386092019 hasConcept C169274487 @default.
- W2386092019 hasConcept C17991360 @default.
- W2386092019 hasConcept C185592680 @default.
- W2386092019 hasConcept C190283241 @default.
- W2386092019 hasConcept C203014093 @default.
- W2386092019 hasConcept C2777527632 @default.
- W2386092019 hasConcept C2780783641 @default.
- W2386092019 hasConcept C48349386 @default.
- W2386092019 hasConcept C503630168 @default.
- W2386092019 hasConcept C53227056 @default.
- W2386092019 hasConcept C54355233 @default.
- W2386092019 hasConcept C55493867 @default.
- W2386092019 hasConcept C62520636 @default.
- W2386092019 hasConcept C71924100 @default.
- W2386092019 hasConcept C81729549 @default.
- W2386092019 hasConcept C86803240 @default.
- W2386092019 hasConcept C91881484 @default.
- W2386092019 hasConceptScore W2386092019C121332964 @default.
- W2386092019 hasConceptScore W2386092019C1491633281 @default.
- W2386092019 hasConceptScore W2386092019C153911025 @default.
- W2386092019 hasConceptScore W2386092019C16685009 @default.
- W2386092019 hasConceptScore W2386092019C169274487 @default.
- W2386092019 hasConceptScore W2386092019C17991360 @default.
- W2386092019 hasConceptScore W2386092019C185592680 @default.
- W2386092019 hasConceptScore W2386092019C190283241 @default.
- W2386092019 hasConceptScore W2386092019C203014093 @default.
- W2386092019 hasConceptScore W2386092019C2777527632 @default.
- W2386092019 hasConceptScore W2386092019C2780783641 @default.
- W2386092019 hasConceptScore W2386092019C48349386 @default.
- W2386092019 hasConceptScore W2386092019C503630168 @default.
- W2386092019 hasConceptScore W2386092019C53227056 @default.
- W2386092019 hasConceptScore W2386092019C54355233 @default.
- W2386092019 hasConceptScore W2386092019C55493867 @default.
- W2386092019 hasConceptScore W2386092019C62520636 @default.
- W2386092019 hasConceptScore W2386092019C71924100 @default.
- W2386092019 hasConceptScore W2386092019C81729549 @default.
- W2386092019 hasConceptScore W2386092019C86803240 @default.
- W2386092019 hasConceptScore W2386092019C91881484 @default.
- W2386092019 hasLocation W23860920191 @default.
- W2386092019 hasOpenAccess W2386092019 @default.
- W2386092019 hasPrimaryLocation W23860920191 @default.
- W2386092019 hasRelatedWork W2047357565 @default.
- W2386092019 hasRelatedWork W2056712531 @default.
- W2386092019 hasRelatedWork W2313400714 @default.
- W2386092019 hasRelatedWork W2349996802 @default.
- W2386092019 hasRelatedWork W2353331129 @default.
- W2386092019 hasRelatedWork W2355963460 @default.
- W2386092019 hasRelatedWork W2369913330 @default.
- W2386092019 hasRelatedWork W2379269741 @default.
- W2386092019 hasRelatedWork W2381665455 @default.
- W2386092019 hasRelatedWork W2383984390 @default.
- W2386092019 hasRelatedWork W2384635545 @default.
- W2386092019 hasRelatedWork W2385705047 @default.
- W2386092019 hasRelatedWork W2391736270 @default.
- W2386092019 hasRelatedWork W2391886397 @default.
- W2386092019 hasRelatedWork W2393198831 @default.
- W2386092019 hasRelatedWork W2411372073 @default.
- W2386092019 hasRelatedWork W2904209929 @default.
- W2386092019 hasRelatedWork W3030467834 @default.
- W2386092019 hasRelatedWork W3032054322 @default.
- W2386092019 hasRelatedWork W3092602616 @default.
- W2386092019 isParatext "false" @default.
- W2386092019 isRetracted "false" @default.
- W2386092019 magId "2386092019" @default.
- W2386092019 workType "article" @default.