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- W2386092233 abstract "Objective:To establish RP-HPLC method for the determination of the content of methionine and vita- min B_1(VB_1)injection and its related substances.Method:The chromatographic column was Lichrosper C_(18)(4.6 mm×250mm,5μm),the mobile phase consisted of water phase(0.216 g sodium hexane sulfonate,2 mL tri- ethylamine,and pH was adjusted to 3.1 with H_3PO_4)-methanol(94:6),at flow rate of 1 mL·min~(-1),detection wavelength was at 210 nm.Results:The linear ranges were 20-200μg·mL~(-1)(r=0.9999)and 2-20 μg· mL~(-1)(r=0.9995)for methionine and VB_1,respectively;the minimal quantifiable level and the minimal detectable level were 100 ng ·mL~(-1)and 30 ng·mL~(-1)for methionine,and 33 ng·mL~(-1)and 10 ng·mL~(-1)for VB_1,respec- tively.Average recoveries of low,medium and high concentration were within 100.2%-101.5%(RSD≤1.4) and 99.0%-99.8%(RSD≤0.75)for methiouine and VB_1,respectively.Conclusion:The method proposed for determinations of the content and its related substances of methionine and VB_1 injection was simple,rapid,specific and accurate." @default.
- W2386092233 created "2016-06-24" @default.
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- W2386092233 date "2007-01-01" @default.
- W2386092233 modified "2023-09-28" @default.
- W2386092233 title "RP-HPLC determination of content of methionine and vitamin B_1 injection and its related substances" @default.
- W2386092233 hasPublicationYear "2007" @default.
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