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- W2386117990 abstract "Objective To investigate the expression of TDAG51 gene in PC12 cells and its function of inducing apoptosis.Methods The experiment was carried out in three groups.In the experimental group,PC12 cells transfected from TDAG51 gene by pEGFP-C 1 carriers were used;in the non-carrier comparison group,PC12 cells transfected from pEGFP-C 1 were used; in the control group,PC12 cells which were not transfected were used.The above celles were collected at 24 h,48 h and 72 h respectively after transfection.Western blotting was employed to detect the expression of TDAG51 protein.The phase contrast microscope was used on 84 h after transfection to observe the change of the morphology of cultured PC12;Using Hoechst 33258 for staining the nucleus and then observe the nuclear morphological change;the change the morphology of nucleolus was observed with Hoechst33258 coloration;the apoptosis implementing protein,Caspase-3,was examined.Results TDAG51 was overexpressed in PC12;compared with the cells in the two comparison groups,the bodies of the cells with TDAG51 overexpression become round and the neurite was lost;Under fluorescene microscope,the nucleolus of PC12 condensed,the chromatin aggregated and nuclear fragmentation could be seen;the pro-caspase-3 protein was activated incised and,the activated 17 kDa Caspase-3 increased more along with the functioning prolongatisn of TDAG51 protein.Conclusion Overexpression of TDAG51 gene can induce the apoptosis of PC12." @default.
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- W2386117990 date "2005-01-01" @default.
- W2386117990 modified "2023-09-23" @default.
- W2386117990 title "Overexpression of TDAG51 gene in PC12 cells and its function of inducing apoptosis" @default.
- W2386117990 hasPublicationYear "2005" @default.
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