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- W2386119304 abstract "A hydrolyzed polyacrylamide(HPAM)-degrading bacteria strain is isolated from curing pot in HPAM distribution station of Daqing oil field using Hungate anaerobic technique.The isolates is characterized morphologically,physiologically,biochemically and molecularly.It is gram negative,short bacillus,non-spore-forming,with an optimum at pH 8.0,with an optimum growth temperature of 40 ℃,strictly anaerobic bacteria,which can reduce sulfate to H2S.Alignment of 16S ribosomal DNA and 16S~23S ribosomal DNA intergenic spacer sequences suggests that this isolate is closely related to the Enterobacter cloacae.The isolate is identified as a new genus belongs to Enterobacter,temporarily named Enterobacter cloacae I7.Analysis results of Infrared Spectroscopy(IR) shows that the bacteria can use HPAM as only carbon source,making HPAM polymer surface structure changed,and hydrolyzing amide to carboxyl group by the molecule chain amido hydrolysis,degrading the side-chain and changing some function group,resulting in obviously decreased viscosity.Using GC-MS,the determined low-molecular weight compounds of HPAM degrading products are polyacrylamide fragments with duplet bond,epoxy as well as carbonyl group,but most of them are acrylamide oligomer derivatives." @default.
- W2386119304 created "2016-06-24" @default.
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- W2386119304 date "2010-01-01" @default.
- W2386119304 modified "2023-09-23" @default.
- W2386119304 title "Isolation and identification of hydrolyzed polyacrylamide (HPAM) degrading bacteria strain Enterobacter cloacae" @default.
- W2386119304 hasPublicationYear "2010" @default.
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