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- W2386133020 abstract "Comparing the denatured PAGE and non-denatured PAGE to establish a simple,rapid,secure,reliable,economic,high efficient and low toxic method of SSR molecular marker,which was applied to genetic diversity analysis of maize.Two microsatellite primer pairs were used to amplify the genomic DNAs of maize.The PCR products were detected by denatured and non-denatured polyacrylamide gel electrophoresis(PAGE).The results of silver-staining indicated that there were many distinct differences about products between the two kinds of gel.The targeted bands clearly appeared in denatured gels,which were easy to identify.There were many nonspecific bands in non-denatured gels.However,targeted bands were very bright,easy to see and did't affect experimental results.The non-denatured gel could reveal genetic diversity analysis among maize inbred lines truly,which was an effective molecular marker to divide germplasm groups of maize." @default.
- W2386133020 created "2016-06-24" @default.
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- W2386133020 date "2013-01-01" @default.
- W2386133020 modified "2023-09-23" @default.
- W2386133020 title "Comparison of Microsatellite Products of Maize Using Denatured and Non-denatured PAGE-Silver-Staining Methods" @default.
- W2386133020 hasPublicationYear "2013" @default.
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