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- W2386142605 abstract "Objective:To prepare complex of carbon nanotubes nerve growth factor(MWCNTs-NGF)through the method of non-covalently grafting,and investigate the bioactivity of the complexes.Methods:We characterized the morphology of the MWCNTs-NGF complex using transmission electron microscopy(TEM),determined the amount of NGF that MWCNTs-NGF complex carried with the method of enzyme-linked immunosorbent assay(ELISA),evaluated the cytotoxicity of the complexes by the method of MTT,evaluated the biological activity of the MWCNTs-NGF complex through cultured pheochromocytoma cells(PC12cells),and characterized the distribution of the complex with the cells using TEM.Results:TEM images showed that NGF was connected to the MWCNTs,the result of ELISA measured the amount of NGF that MWCNTs-NGF complex carried was 797.63pg·mg,MTT tests showed that the MWCNTs-NGF complex have some toxicity on PC12cells,biological activity tests showed that the differentiation of MWCNTs-NGF complex group on PC12cells was significantly higher than the NGF group in the case of the same concentration of NGF,and TEM images showed that carbon nanotubes could enter cells.Conclusion:Carbon nanotubes can carry NGF protein into the cells and help to promote nerve cell growth." @default.
- W2386142605 created "2016-06-24" @default.
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- W2386142605 date "2013-01-01" @default.
- W2386142605 modified "2023-09-24" @default.
- W2386142605 title "Preparation and Bioassay of Multi-Walled Carbon Nanotubes-Nerve Growth Factor Complex" @default.
- W2386142605 hasPublicationYear "2013" @default.
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