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- W2386144486 abstract "Objective To establish the protocol for culturing hippocampus neurons in vitro and explore the neuronal growth character istics.Methods Tissues from hippocampus of fatal rats were harvested,then digested into cell suspension by using 0.125% trypsin.After planted into wells for 2 hours,cell suspension was transferred into next well for continuing incubation.The cell growth was observed at day 5,11 and 15.Neurons specific Enolase(NSE)antibody,recognized specifically NSE antigen in cultured cells,was used to identify neurons by SP-two-step staining method.Results After incubated for 2 hours,transferred suspensions in wells showed some pure neurons,which is identified by NSE staining.However,they grew slowly during 1～3 days,then grew well from 5th day,with gradual increasing the neuritis outgrowth.Conclusion Purified neurons with neurite outgrowth states can be acquired by this method." @default.
- W2386144486 created "2016-06-24" @default.
- W2386144486 creator A5009257635 @default.
- W2386144486 date "2011-01-01" @default.
- W2386144486 modified "2023-09-25" @default.
- W2386144486 title "Identification and Culture of Hippocampus Neurons in Fetal Rats" @default.
- W2386144486 hasPublicationYear "2011" @default.
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