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- W2386145181 abstract "Objective:To construct and analyze protein activity of microneme protein 6(MIC6) mutant in Toxoplasma gondii.Method:The tryptophan(W) at sites 348 of carboxyl terminus of MIC6(MIC6C) was mutated into valine(V) by site-directed mutagenesis,MIC6C W/V gene was obtained from cDNA library by PCR amplification and subcloned into pGEX-4T-1.The mutant protein GST-MIC6C W/V was expressed upon IPTG induction.Glutathione sepharose beads was incubated with GST-MIC6C W/V,then incubated with tachyzoite lysates,and bound proteins were eluted using sample buffer.Eluants were resolved by SDS-PAGE and Western blot.Result:MIC6C W/V gene was amplified and the mutant protein GST-MIC6C W/V was obtained; Protein band specifically recognized by anti-aldolase antibody was detected in products coming from GST pull-down of GST-MIC6C,but not in pull-down products coming from GST-MIC6C W/V.Conclusion:The mutant of MIC6 was prepare in vitro; MIC6 mutant protein lost the binding ability to aldolase." @default.
- W2386145181 created "2016-06-24" @default.
- W2386145181 creator A5047307441 @default.
- W2386145181 date "2014-01-01" @default.
- W2386145181 modified "2023-09-23" @default.
- W2386145181 title "Construction and Analysis Protein Activity of MIC6 Mutant in Toxoplasma gondii" @default.
- W2386145181 hasPublicationYear "2014" @default.
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