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- W2386185320 abstract "The purpose is to discuss the influences of TGF-β 1 on biological behaviors of tumor cell by comparing the biological behaviors like cell adhesion and migration of different human lung carcinoma cell. The methods used in the study are that different human lung carcinoma cells are maintained in RPMI1640 supplemented with penicillin/streptomycin, 10% heat inactivated fetal bovine serum (FBS) in 37℃ and 5% CO 2. We have designed two assays (in vitro wound healing assays and agarose drop explants method) to evaluate the migration and to evaluate cell adhesion according to Dean Sheppard's method. Cell spreading and proliferation are studied too. The results show that the ability of 95 C migration and the rate of its cell spreading and proliferation is at the top; 95D is in the second place. The cell adhesions onto Fibronectin (Fn) following TGF-β 1 treatment in 95C and 95D cells are increased as compared to that of control, and all cells migration is promoted by 24 and 48hr TGF-β 1 treatment. It suggested that there are differences of cell adhesion and migration among different human lung carcinoma cells. TGF-β 1 may be involved in the regulation of integrin-mediated signal transudation, which may influence the malignant behavior of human lung carcinoma cell lines." @default.
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- W2386185320 date "2002-01-01" @default.
- W2386185320 modified "2023-09-22" @default.
- W2386185320 title "Effect of TGF-#beta#_(1) on biological behavior of different human lung carcinoma cell in vitro" @default.
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