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- W2386197311 abstract "【Aim】Comparative analysis was performed to investigate differentially expressed proteins between non- and summer-diapausing pupae of the onion fly,Delia antiqua,and to provide a theoretical basis for further exploring the molecular regulation mechanisms of insect diapause and pest control management. 【Methods 】 Total proteins were extracted from non- and summer-diapausing pupae, separated by two-dimensional gel electrophoresis,and then analyzed by image analysis. Peptide mass fingerprinting of differential proteins were analyzed by MALDI-TOF MS and identified through database searching in NCBI and SWISS PORT using MASCOT. 【Results】Proteins were differentially expressed in non- and summer-diapausing pupae. Thirteen differentially expressed proteins were identified by mass spectrometry and bioinformatic analysis,including collagen,SAS6 (spindle assembling abnormal protein 6),MTHFS (5,10-methenyltetrahydrofolate synthetase),Bnb (bangles and beads) and proteins with unknown functions. 【Conclusion】The expressions of some proteins are up-or down-regulated in summerdiapausing pupae. Proteins identified in this study may be members of diapause-related protein regulatory networks, and they potentially play important roles in high temperature resistance, chromosome separation,folate metabolism and other physiological processes." @default.
- W2386197311 created "2016-06-24" @default.
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- W2386197311 date "2014-01-01" @default.
- W2386197311 modified "2023-09-26" @default.
- W2386197311 title "Comparative analysis of proteins in non- and summer-diapausing pupae of the onion fly,Delia antiqua (Diptera: Anthomyiidae ),using twodimensional gel eletrophoresis" @default.
- W2386197311 hasPublicationYear "2014" @default.
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