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- W2386221812 abstract "The extraction of alkali-soluble proteins from Juglans mandshurica Maxim kernel waste left over after oil extraction with supercritical carbon dioxide was optimized using one-factor-at-a-time and orthogonal array design methods.The effects of NaOH concentration,material-to-solvent ratio,temperature and extraction time on the extraction yield of protein.It was found that the highest extraction yield of protein,88.05%,was obtained after 1.5 h of extraction with 0.02 mol/L NaOH solution at a material-to-solvent ratio of 1:30 and 50 ℃.The isoelectric point of the extract obtained under these conditions was measured to be 4.5.SDS-PAGE analysis showed that the molecular weight distribution of alkali-soluble proteins from Juglans mandshurica Maxim kernels was mainly 100,60,36 kD and 20 kD." @default.
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- W2386221812 date "2012-01-01" @default.
- W2386221812 modified "2023-09-23" @default.
- W2386221812 title "Extraction and SDS-PAGE Analysis of Alkali-Soluble Proteins from Juglans mandshurica Maxim Kernels" @default.
- W2386221812 hasPublicationYear "2012" @default.
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