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- W2386253592 abstract "Objective: To identify the differential expression genes of the regenerating somatosensory cortex after the spinal cord of the newborn rats was hemi-sectioned, to clone them and analysis their relationship with regeneration.Method: The regeneration model of the newborn rats whose bilateral spinal cord were hemi-sectioned was used. The fetal spinal cord tissue was transplanted at their unilateral lesion site. The suppression subtractive hybridization method was used to screen the differential expression genes of motor cortex neuron of the newborn rats. The differential expression library was constracted. The blue/white spot test and dot hybridization technique was used to certificate the really positive clones. Then their sequences was analysed. Result: 25 novel ESTs were discovered and got accession in GenBank.Conclusion:The somatosensory cortex neuron of the newborn rats with transplanted fetal spinal cord have a differential expression of the regeneration relation genes, the differential genes may influence the regeneration reaction.Author′s address Research Institute of Surgery, Third Military Medical University, Chongqing 400042" @default.
- W2386253592 created "2016-06-24" @default.
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- W2386253592 date "2004-01-01" @default.
- W2386253592 modified "2023-09-24" @default.
- W2386253592 title "Clones screening and sequence identification of regeneration relation gene in newborn rats by suppression subtractive hybridization" @default.
- W2386253592 hasPublicationYear "2004" @default.
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