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- W2386312709 abstract "A novel diagnostic method was established to detect the presence of Eperythrozoon suis,two pairs of primers to Eperythrozoon suis were successfully designed according to the 16S rRNA gene sequence of E.suis from GenBank(U88565),and to develop a single-tube nested PCR assay for detection of E.suis.The cloned genes demonstrated by analysis with enzyme digestion and the single tube nested PCR were proved to be the specific genes for E.suis and the sequence of clone E.suis was compared with that of E.suis published in GenBank (U88565).Meanwhile,staining of blood smear and conventional PCR were compared with the single-tube nested PCR of E.suis.The results showed that the sequence of the clone E.suis was 96% homology with U88565.As demonstrated by the specificity testing,the PCR primers designed in the present study could not be amplified to form bands from the genomic DNA of Toxoplasma gondii,Streptococcuss species,E.coli,Staphylococcus species and Mycoplasma ovis.The lowest value of sensitivity of the single-tube nested PCR was 0.116 fg DNA of the standard template.The detection of 75 blood samples showed that the a single-tube nested PCR with high sensitivity and practicability is obviously superior to the staining of blood smear and conventional PCR for the detection of Eperythrozoon suis." @default.
- W2386312709 created "2016-06-24" @default.
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- W2386312709 date "2011-01-01" @default.
- W2386312709 modified "2023-09-26" @default.
- W2386312709 title "Establishment and application of a single-tube nested PCR assay for detection of Eperythrozoon suis" @default.
- W2386312709 hasPublicationYear "2011" @default.
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