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- W2386316589 abstract "【Objective and Method】The total RNA template was isolated from the nasal secretion of clinically infected dogs with canine parainfluenza virus(CPIV).According to the sequence of CPIV published in GenBank,a pair of primers were designed in NP gene region and the specific 667bp nucleotides band was found on agarose electrophoresis.The RT-PCR method for detection of CPIV was established by this way.【Result】The specific test found the primers could only amplify the genome of CPIV,rather than canine distemper virus,canine parvovirus,canine adenovirus and rabies virus.As little as 10~(-6) total RNA template of CPIV could be detected in the sensitive test.The reproducibility for the RT-PCR was highly stable.53.12% of positive cases were found in 32 samples by RT-PCR.【Conclusion】The established RT-PCR method in this paper could be used in the early clinical diagnosis and the small-scale of epidemiological survey for CPIV infections in dogs." @default.
- W2386316589 created "2016-06-24" @default.
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- W2386316589 date "2011-01-01" @default.
- W2386316589 modified "2023-09-28" @default.
- W2386316589 title "Foundation and Application of RT - PCR Method to Detect Canine Parainfluenza Virus in Pet Dogs" @default.
- W2386316589 hasPublicationYear "2011" @default.
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