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- W2386345625 abstract "【Objective】The special expression vector pPB-AFABP of A-FABP gene,containing piggyBac(PB)as transposon component,green fluorescence protein(EGFP)as reporter gene,Neomycin(NEO)as resistance gene,andα-actin protein as promoter,was constructed and the transposition activity was verified.【Method】The transgene vector pPB-AFABP of A-FABPgene sequence was connected with PB transposon backbone sequence,NEO-EGFP sequence,andα-actin promoter sequence.These sequences were synthesized by PCR amplification.PB binary system consisting of the plasmid pPB-AFABP and helper plasmid pCAG-PBase and vector pPB-AFABP were transfected into bovine fibroblasts by liposomes,respectively.The transgenic cells after screened by G418 was observed.【Result】The final transgene vector pPBAFABP was verified by restrictive enzyme and sequencing.The number of the positive colonies in bovine fibroblasts transfected with PB binary system was larger than that transfected with the plasmid pPB-AFABP alone.PCR results showed that the A-FABP gene existed in bovine transgenic fibroblast.【Conclusion】A transgene vector pPB-AFABP was successfully constructed,and a high transposition activity of the PB transposon in bovine fibroblasts was verified." @default.
- W2386345625 created "2016-06-24" @default.
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- W2386345625 date "2013-01-01" @default.
- W2386345625 modified "2023-09-28" @default.
- W2386345625 title "Construction and identification of expression vector of A-FABP mediated by piggyBac transposon" @default.
- W2386345625 hasPublicationYear "2013" @default.
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