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- W2386368848 abstract "To investigate whether the blaNDM-1 gene was carried by drug-resistant bacteria in the farms and farmer′s markets,the PCR primers were designed according to the sequence of blaNDM-1 gene from GenBank and the PCR system and reaction conditions were optimized.The specificity,sensitivity,repeatability of PCR detection method for blaNDM-1 gene was verified by positive control of synthetic blaNDM-1 gene and negative control of 4 reference strains including of E.coli,Klebsiella pneumoniae,Enterobacter cloacae and Pseudomonas aeruginosa.229 isolates of E.coli and 31 isolates of Streptococcus were detected by PCR method.The results showed that the specificity,sensitivity,repeatability of PCR method for blaNDM-1 gene was established and the target gene was about 241bp.The minimal detectable DNA concentration was 9.18×10-7 μg/μL.There was no target fragment on agarose gel for 229 isolates of E.coli and 31 isolates of Streptococcus.It showed that blaNDM-1 gene was not carried by bacterial isolates from some area of Guangdong province.The PCR method can be used on monitoring of drug-resistant bacteria as an early diagnosis and rapid screening method for the drug-resistant bacteria carrying blaNDM-1 gene in veterinary clinic." @default.
- W2386368848 created "2016-06-24" @default.
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- W2386368848 date "2013-01-01" @default.
- W2386368848 modified "2023-09-24" @default.
- W2386368848 title "Establishment and Application of PCR for Detection of Bacterial bla_(NDM-1) Gene" @default.
- W2386368848 hasPublicationYear "2013" @default.
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