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- W2386372215 abstract "For detection of Muscovy duck parvovirus(MDPV) infection,a real-time PCR assay was established with a pair of primers and a TaqMan probe which were designed and synthesized according to the conserved REP gene sequences of MDPV.The real-time PCR assay was specific to detect MDPV,but no cross-reaction with duck hepatitis virus type I,duck circovirus,gosling plague virus,duck paramyxovirus,duck plague virus and avian influenza virus.In addition,the detection limit of this assay was 20 viral copies which was 100 times higher than that of the conventional PCR.The coefficients of variability for intra-assay and inter-assay were both less than 2%.Furthermore,detection of 118 duck clinical samples shown that the positive rate was 11.02%.It suggested that the real-time PCR could be used as a rapid tool for detection of MDPV." @default.
- W2386372215 created "2016-06-24" @default.
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- W2386372215 date "2013-01-01" @default.
- W2386372215 modified "2023-09-23" @default.
- W2386372215 title "Development and application of a real-time PCR assay for detection of Muscovy duck parvovirus" @default.
- W2386372215 hasPublicationYear "2013" @default.
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