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- W2386389613 abstract "Objective To investigate the possibility of poly hydroxybutyrate-co-hydroxyvalerate (PHBV) as scaffolds for chondrocytes. Methods PHBV porous scaffolds were fabricated using a compression moulding,thermal processing and salt particulate leaching method. The porous structure of the scaffolds was investigated with optical microscope (OM) and scanning electron microscope (SEM) and the porosity was calculated. Chondrocytes isolated from articular cartilage were seeded into PHBV scaffolds and cultured in vitro. After incubation for 3,7 and 14 days,the chondrocytes on the scaffolds were examined by SEM,and the cell-scaffolds cultured in vitro for 2,6 and 10weeks were examined histologically. Results The pore size in the scaffolds ranged 200~300μm and the porosity was about 80%. During the culture periods,the specimens maintained their original shapes. SEM showed that the chondrocytes could aggregate and synthesize extracellular matrix on PHBV scaffolds. Histological examination confirmed that morphology of the chondrocytes and the expression of the cartilaginous matrices and characteristics of the engineered cartilage generated in vitro were more typical with increased culture period. Conclusion PHBV scaffold is suitable in cartilage tissue engineering as it has optimal porous structure and biocompatibility." @default.
- W2386389613 created "2016-06-24" @default.
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- W2386389613 date "2006-01-01" @default.
- W2386389613 modified "2023-09-22" @default.
- W2386389613 title "Experimental study on PHBV as a scaffold in culture of chondrocytes" @default.
- W2386389613 hasPublicationYear "2006" @default.
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