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- W2386411177 abstract "Objective:To determine the contents of 18α-glycyrrhizic acid and 18β-glycyrrhizic acid in Diammonium Glycyrrhizinate Injection by RP-HPLC. Methods:The Chromatographic method was carried out on ZORBAX Extend C18 column using acetonitrile-phosphate buffer solution(pH=7∶20∶80 ) as a mobile phase. The detection wavelength at 250nm.The flow rate was 1mL·min-1.The column temperature was 30℃. Took control solution mixture and experimental solution, poured in chromatograph of liquid respectively, with peak area external reference method of 18α-glycyrrhizic acid and 18β-glycyrrhizic acid in control solution mixture chromatogram, to determined the contents of 18α-glycyrrhizic acid and 18β-glycyrrhizic acid.Results:The 18α-glycyrrhizic acid and 18β-glycyrrhizic acid calibration curves were linear at the of 0.1010~ 1.0100μg(r=0.99984)and 0.1033~1.0330μg(r=0.99948).The average recoveries were 98.02% with RSD 0.35% for 18α-glycyrrhizic acid and 97.5% with RSD 0.54% for 18β-glycyrrhizic acid.Conclusion:This method is simple, reliable and provides a reference standard for the quality control of Diammonium Glycyrrhizinate Injection." @default.
- W2386411177 created "2016-06-24" @default.
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- W2386411177 date "2008-01-01" @default.
- W2386411177 modified "2023-09-26" @default.
- W2386411177 title "Determination of 18α-Glycyrrhizic Acid and 18β-Glycyrrhizic Acid in Diammonium Glycyrrhizinate Injection by RP-HPLC" @default.
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