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- W2386415132 abstract "Objective To explore the influence of epidermal growth factor (EGF) on the growth of murine extrahepatic bile duct epithelial cells (MEBECs) in vitro. MethodsThe extrahepatic bile ducts of BALB/c mice were digested with trypsin in the presence of DNaseⅠand further digested with collagenase Ⅳ. MEBECs were isolated and seeded in a culture flask containing DMEM/Hams F12 (1∶1) supplemented with or without 10 ng/mL epithelial growth factor (EGF) respectively. Then the morphologic changes of the cells were observed with inverted phase contrast microscope and MTT assay detected the influence of EGF on the growth of BECs. ResultsThe duration of the MEBECs of the group with EGF was obviously longer than the other group (12.1±2.1 d vs 21±1.3 d, P 0.001). And the MEBECs of the group with EGF had better cell vigor (P 0.001).ConclusionEGF added to medium can obviously promote the proliferation of MEBECs and prolong the duration of MEBECs in vitro." @default.
- W2386415132 created "2016-06-24" @default.
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- W2386415132 date "2010-01-01" @default.
- W2386415132 modified "2023-09-23" @default.
- W2386415132 title "Experimental research of the influence of epidermal growth factor on murine extrahepatic bile duct epithelial cells primary culture" @default.
- W2386415132 hasPublicationYear "2010" @default.
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